4/8-4/12 & 4/15-4/19 Conference Preparation and Future Plans

I feel as though it is appropriate to combine these two weeks into one blog post since during these weeks we had not done much in terms of our project in terms of procedures. During the first week we were mainly focused on getting our poster ready for the Arizona Nevada Academy of Science conference that was on 4/13. So, we were designing our poster throughout the majority of the week to make sure that our poster was as professional as possible and appropriately displayed our findings from the last few weeks that we had spent working with D. Metalli. Working on the poster and preparing for the conference took a majority of our time in the first week so we didn’t do any further procedures on D. Metalli and its ability to grow in R2A to observe for biofilm formation. 

Our Poster on D. Metalli Characterization at the Arizona Nevada Academy of Science


 In the second week there was not much that was done as well, somewhat due to us not really sure on how we wanted to move forward. This is because we had the option of moving forward on two different projects, one being the Gibson Assembly project that we had been working on for several months, and the other being the D. Metalli project. Slowly throughout the week we started to gather materials for both projects, as in we would make some R2B to observe growth of D. Metalli biofilm in a broth that was not nutrient rich. For the Gibson project we had started to make some LB Ampicillin plates and a few TGY plates. The LB Amp plates were for the fully assembled plasmid to see if the plasmid assembled correctly, which if so would insert ampicillin resistance to the specific E. Coli we would be transforming. 

So this second week was more of deciding how to move forward with both projects and then slowly gathering the materials needed for both projects. 

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